Abstract
Summary The aim of this study was to evaluate antioxidant, antiproliferative and antimicrobial properties of flying fish (Exocoetus volitans) backbone hydrolysed by three different enzymes namely papain, pepsin and trypsin. The in vitro antioxidant potencies of hydrolysates and purified peptides against 1, 1-diphenyl-2-picrylhydrazyl, superoxide and hydroxyl radicals were evaluated by electron spin resonance spectroscopy. The peptic protein hydrolysate showed maximum free radical scavenging potential and lipid peroxidation inhibition and was further purified by DEAE XK 26/20 anion exchange chromatography followed by G-25 gel permeation chromatography. The amino acid composition of potent purified fraction was determined by HPLC, contains essential and nonessential amino acids with glutamic acid (24.10%), lysine (23.62%), glycine (12.05%) and threonine (10.41%) as the dominant amino acids. The potent purified fraction was tested for cell cytotoxicity on Vero and Hep G2 cell lines. It was found that fraction IIIb did not show any cytotoxic effect for Vero cell lines and exerted a significant antiproliferative effect on Hep G2 cell lines.
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