EVALUATION OF BEDSIDE PROTHROMBIN TIME AND ACTIVATED PARTIAL THROMBOPLASTIN TIME MEASUREMENT BY COAGULATION ANALYZER COAGUCHECK PLUS® IN VARIOUS CLINICAL SETTINGS

Abstract

In the present study CoaguChek Plus ® (CCP ®), a coagulation test system using whole blood, was evaluated with respect to its comparability with widely distributed conventional routine coagulation assays. A correlation of r=0.997 (p<0.0001) was found between INR of CCP ®-prothrombin time (CCP ®-PT) and Thrombotest ® (KC-1 analyzer) in patients on oral anticoagulant therapy. A correlation of r=0.899 (p<0.001) between CCP ®-aPTT and Actin FS ® aPTT (STA analyzer) was found in heparinized patients. Impaired hepatic coagulation factor synthesis in liver cirrhosis patients was detected by CCP ®-PT with a sensitivity of 0.75 and by Normotest ® (STA analyzer) with a sensitivity of 0.92. Those patients with normal CCP ®-PT values and liver disease had, only mild reductions (> 30% of normals) in coagulation factors II, V, VII or X. CCP ®-aPTT was also performed in patients with a deficiency in the so called endogenous coagulation factors VIII, IX, XI and XII. CCP ®-aPTT showed a sensitivity similar to that of Actin FS ® aPTT in the detection even of mild deficiencies in factors VIII, IX and XII; factor XI deficiency was however detected only in patients with severe (<12% of normals) disease; lupus anticoagulants were detected with a high sensitivity. © 1997 Elsevier Science Ltd