Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

Abstract

The aim of this research was to evaluate the Bali cattle semen quality during cryopreservation with different types of extenders in term of live, total motility, progressive motility, and abnormality of post-thawed bull sperms. The treatments were AndroMed® (T0), Tris-based egg yolk diluent (T1), Tris-based coconut water diluent (T2), Coconut water egg yolk diluent (T3). Bulls’ semen was collected from two adult Bali cattle maintained at the semen production facility at Bali Artificial Insemination Center, Tabanan Bali. The age of the bulls were 6 years old. Sperm live, total motility, progressive motility, and abnormality were analyzed with computer assisted sperm analysis (CASA) post-dilution, before and after thawed. The study was replicated five times, and data were analyzed using analysis of variance (ANOVA). The results showed that AndroMed ® and Tris-based egg yolk had significantly higher sperm live, total motility, progressive motility and abnormalities of spermatozoa for post-dilution, after equilibration and post thawed than Tris egg yolk coconut water and Coconut water egg yolk diluent. It was concluded that AndroMed® and Tris-based egg yolk can be considered as the best suitable extender for Bali cattle sperm cryopreservation. Coconut water had a deleterious effect when supplemented with 20% in tris and egg yolk.