Evaluation of Bacterial Nanocellulose Membranes Loaded or Not with Nisin as a Complementary Treatment in Surgical Dehorning Wounds in Bovines.

Fábio A F Custódio,Alessandre Hataka,Ana Carolina R C Porto,Iolanda S Braga,Angela F Jozala,Denise Grotto,Erick Unterkircher,Leonardo M De Castro

doi:10.3390/pharmaceutics13050688

Fábio A F Custódio, Alessandre Hataka + Show 6 more

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https://doi.org/10.3390/pharmaceutics13050688

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Abstract

Treatments of postsurgical dehorning in cattle usually includes topical application of healing agents in wounds. The Bacterial Nanocellulose (BNC) may come to a complementary treatment for these wounds. Two new complementary treatments with BNC and BNC loaded with nisin were evaluated for wound healing in surgical dehorning in bovine. Hence, two groups of experiments were run, and 12 animals were used in each group. All animals, in right and left horns, received antisepsis treatment. For the first group, the left horn was the control, and on the right one the BNC was applied. For the second group, BNC was applied on the left horn (control) and on the right ones BNC+nisin was applied. In both experiments, wounds were evaluated macroscopically by photographic images and microscopically by histology. For macroscopic evaluations, a significant difference was observed over time, but only in the comparison within the same groups. Microscopic analyzes did not showed significant differences in any type of comparison. In conclusion, there was a clinical improvement in the wound healing response with the application of BNC. However, there was no significant difference between BNC compared to the nisin loaded in BNC. For the first time it was applied a BNC in surgical dehorning wounds in bovines and evaluated the efficacy of treatment in a real animal handling situation.

Highlights

IntroductionWelfare practices include animal handling, i.e., the procedures to which animals are subjected to achieve the desired production efficiency
On day 10 (Figure 1C), there was a decrease in the healing process in Control, as 83% of the animals were within score 3, compared to 33% of Bacterial Nanocellulose (BNC) in score 4
The concentration used was 25 μg/mL, a fact that corroborates the results found by the cited authors. It was performed the application of BNC as a dressing matrix and as a drug release for nisin in surgical dehorning wounds in bovines, followed by evaluating the efficacy of the BNC treatment in a real breeding environment

Summary

Introduction

Welfare practices include animal handling, i.e., the procedures to which animals are subjected to achieve the desired production efficiency. Some surgical procedures are performed to promote AW, for productive gain, and to ensure safety for handlers [1], since the procedures contribute to reducing the intensity of aggressive interactions among animals [2,3]. Surgical dehorning in adult animals is a procedure performed in cases of aggressiveness among animals, fractures, or abnormal conformation of the horns, despite being an invasive procedure [4]. Dehorning contributes to reducing the frequency of lesions in udders, eyes, and flank region [5], besides promoting greater safety for handlers [1,6]

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