Evaluation of bacterial behavior in capillary vessel size micro-channel

Abstract

Toward to realizing establishment of new micro robot for micro-nano surgery, drag delivery etc., many people proposed microbe as actuator. So, evaluation of microbe property for these application arias is important. In this paper, we try to fabricate evaluation tool for bacterial behavior in micro channel like capillary vessel. For this purpose, we fabricated line patterned PDMS chip and use these chip as micro channel. Line pattern was fabricated on F-template by nano imprinting system, and these patterns were transferred on polydimethylsiloxane (PDMS). And then some kind of marine bacterial cells (Vibrio alginolyticus), this bacteria can swim in water, are injected into the fabricated micro channel and observed their behavior. As first step, we observed relationship between channel width and swimming behavior, and we found that 1µm channel limited cell behavior as one-dimensional motion.