Evaluation of apical bacterial leakage of three different obturation systems after using two different irrigating solutions: An in vitro study

Abstract

Aim: The present study aimed to compare between apical bacterial leakage of obturating systems such as EndoRez, ActiV GP and AH Plus sealers using gutta-percha, with two different irrigating materials Biopure MTAD (mixture of doxycycline, acetic acid and detergent) and 2.25% sodium hypochlorite (NaOCl). Methods: Seventy extracted human mandibular premolars with single canal were divided into two equal groups of 30 samples each according to the irrigating solution used either Biopure MTAD or 2.25% NaOCL. The other 10 teeth (5 roots positive group and 5 roots negative group) were used as control. The main groups were subdivided into 3 subgroups according to the obturation system used, EndoRez, ActiV GP or AH Plus with gutta percha. Sealing ability was assessed using Enterococcus faecalis (E. faecalis) in a double chamber leakage model. Samples were monitored weekly for periods of 1, 4 and 8 weeks to assess the turbidity of the medium in contact with the lower coronal portion of the root segment. Results: Showed statistically significant increase in the mean apical leakage with specimens irrigated with 2.25% NaOCL than Biopure MTAD in all periods, while roots obturated with EndoRez showed less statistically significant leakage than the rest of the experimental materials at 1 and 4 weeks periods while, at 8 weeks period all the experimental materials showed leakage with no statistically significance difference. Conclusion: EndoRez when used after Biopure MTAD irrigation provides better sealing with root canal dentin.