Evaluation of antiurolithiatic and antioxidant activity of the Egyptian Pluchea dioscoridis L. leaves extracts in vitro

Abstract

Bioactive compounds in medicinal plants are believed to be an important in the folk of medicine. Many efforts have been done to discover the safe medical treatment instead of chemical drugs which have a harmful effect on the human body. In the present study the crude extracts of Pluchea dioscoridis were tested to evaluate their bioactive compounds (total phenolic and flavonoid compounds) and their effects as antiurolithiasis of human stones (calcium triphosphate (CTP), cholesterol and calcium oxalate monohydrate (COM) besides, their antioxidant activities. The measured total phenolic compounds content in the methanol, ethyl acetate, and water extracts of Pluchea dioscoridiswere found to be equal to 11.842, 9.047, and 8.126 mg/g, respectively. Also, the contents of the total flavonoid compounds (total phenol) were 19.018, 15.312, and 14.022mg/g for methanol, ethyl acetate, and aqueous extracts of P.dioscoridis, respectively. The results showed that all extracts of P. dioscoridis have a significant inhibitory effect on the dissolution of the three investigated types of formed stones (CTP, cholesterol, and COM). The inhibition effect was in the order methanol > ethyl acetate > water extract. Also, it was found that all extracts for P.dioscoridis were exhibited 2, 2, diphenyl-1-picryl hydrazil (DPPH) radical, reducing power, ABTS∙+ radical, Nitric oxide radical scavenging activity. Methanol extract of P. dioscoridis exhibited the highest scavenging activity compared to ethyl acetate and water extracts. Refaat Ahmed Saber