Evaluation of Antioxidant Potential of Asystasia Chelonoides var.chelonoides Nees.(Acanthaceae)

Abstract

The ethanolic leaf extract of Asystasia chelonoides var. chelonoides Nees. was evaluated using various in vitro and ex vivo assays. Evaluations were made for DPPH radical scavenging activity, NO radical scavenging activity, Super oxide radical scavenging activity ,Ferric Reducing Antioxidant Potential (FRAP) assay, Total antioxidant activity , Reducing Power activity and Lipid Peroxidation inhibition. Over all findings revealed that A.chelonoides leaf exhibited four extracts namely (AC HEX, AC CHL, AC ETH and AC CRD) and were selected for the study, AC ETH and AC CRD showed potent antioxidant activity by inhibiting DPPH radical in a concentration dependent manner . The nitric oxide radical scavenging activity of all the samples increased as their concentration increased from 20 µg/mL to 320 µg/ mL. AC CRD showed its maximum superoxide radical scavenging of 78.56 % at 320 µg/mL and the EC50 was found to be 93.26 µg/mL. The extracts exhibited a dose dependent reducing power activity within the applied concentrations (20 µg/mL to 100 µg/mL) and AC CRD at 100 µg/mL concentration showed highest activity, which was almost comparable to standard ascorbic acid. In FRAP assay the crude extract of A. chelonoides (AC CRD ) showed 138.14 µg trolox equivalent/g of dry extract respectively. Total antioxidant activity of AC CRD, AC HEX, AC CHL and AC ETH were found to be 98.27, 14.86, 20.16 and 72.56 µg of ascorbic acid equivalent/mg of dry extract respectively. A.chelonoides crude extract and ethanolic fraction showed potent inhibition of FeCl2AA stimulated rat liver lipid peroxidation in concentration dependent manner.