Abstract
Marine microalgae are a photosynthetic organism which can produce diverse metabolites, biomass and O2. The bioactive compounds produced by microalgae includes polysaccharides, polyunsaturated fatty acids, polyphenolic compounds, antioxidants, peptides, essential vitamins and minerals. The present study aimed to evaluate the antioxidant activity and bioactive metabolites of the marine microalgae Nannochloropsis sp. Various solvent (petroleum ether, aqueous, isopropanol, methanol and ethyl acetate) extract of Nannochloropsis sp. were evaluated for antioxidant activity. The ethyl acetate extract contributes maximum total antioxidant activity of 71.35 ± 0.04 mg of AAE/g of extract. The ethyl acetate extract exhibited highest on nitric oxide scavenging metal chelating and reducing power assay with % inhibition of 79.57 ± 0.215, 80.73±0.105, 0.64±0.001 at 1mg/concentration respectively. Methanolic extract showed hydroxyl radical scavenging activity of 78.72±0.21 % inhibition at a concentration of 1mg/ml. The ethyl acetate extract with highest antioxidant activity was analyzed for bioactive metabolites by GCMS. Among the 15 compounds obtained through GCMS, the major component n-Hexadecanoic acid was reported to have antioxidant activity. The results from the different types of extracts clearly indicated that the bioactive components present in the microalgae contribute significantly to the antioxidant capacity of microalgae.
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