Evaluation of Antioxidant Interactions in Combined Standardized Extracts of Tinospora Cordifolia (Willd), Terminalia Arjuna (Roxb) and Ocimum Sanctum (LINN)

Abstract

Generation of reactive oxygen species ROS occurs in the normal cell during metabolism in animals. Over production of ROS in the animals causes oxidative stress that leads to oxidative DNA damage which is implicated in the pathogenesis of numerous diseases. Since antioxidant mechanism plays vital role in the pharmacotherapy of different disorders the present investigation aims to check the synergisticantagonistic antioxidant effects of standardised extracts of Tinospora cordifolia Willd TCE Terminalia arjuna Roxb TAE Ocimum sanctum Linn OSE in combinations by DPPH Scavenging Activity hydroxyl radical scavenging activity reducing power assay. In all the methods the extract combinations exhibited good scavenging activity. Reaction of combination of standardized extracts with 22 Diphenyl -1-picryl hydrazyl DPPH radicals showed better scavenging activity compared to individual extracts. The IC50 values for TCETAE TCEOSE TAEOSE were found to be 74.12 microg ml 78.62 microg ml and 71.70 microg ml respectively. The IC50 values for hydroxyl radical scavenging activity of different herb combinations viz. TCETAE TCEOSE TAEOSE were found to be 320.28 microgml 340 microgml and 261 microgml respectively. Further the standardized herb combinations showed more effective reductive ability when compared to that of individual standardized herbal extract. Hence it may be concluded that standardized extracts containing potent antioxidant constituents like phenolics flavonoids triterpenoids alkaloids steroids tannins showed synergistic antioxidant activity.nbsp