Abstract
Despite widespread use of Khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (KGA) in the traditional medicine, there was a lack of scientific evidence on its efficacy and safety. The present investigation was designed to evaluate its ex vivo antioxidant and in vivo hepatoprotective properties against carbon tetrachloride toxicity in albino rats. At first phytochemicals analysis of test preparation was conducted to estimate its total phenolic and flavonoid contents. Then their antioxidant activity was determined by various tests and compared it with standards ascorbic acid and rutin. Afterwards, hepatoprotective activity was studied against carbon tetrachloride-induced liver damage by determining SGOT, SGPT, ALP, total cholesterol, bilirubin and total proteins contents in the serum of rats before and after treatment. This suggests that the hepatoprotective activity of formulation is possibly attributed to its free radical scavenging properties.
Highlights
In South East Asia region, many herbs are used in different combinations in the preparations of patent herbal formulations (Sharma et al, 1991)
The polyherbal formulation of KGA manufactured by Hamdard Laboratories, Karachi, a reputed Unani Pharmaceutical concern was purchased from local market of Sargodha, Pakistan
Folin‐Ciocalteu reagent, diagnostic kit, ascorbic acid, rutin, silymarin, carbon tetrachloride and other chemicals were purchased from Merck, Germany
Summary
In South East Asia region, many herbs are used in different combinations in the preparations of patent herbal formulations (Sharma et al, 1991). A polyherbal hepatoprotective formulation Khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (abbreviated here as KGA) was selected for pharmacological evaluation. The criteria for selection included: i) claims in traditional medicine, ii) reports of presence of flavonoids and flavones contained in the constituent plants, iii) semi-solid formulation easy for oral administration, iv) established hepatoprotective activity of one or more plants, and v) sufficiently long shelf life. This herbal preparation has been traditionally used since centuries for treatment of various hepatic diseases and is considered curative and even hepatoprotective, nervine, general and cardiac tonic
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