Abstract
Objective: The objective of the present research work had been made to evaluate the antioxidant potential along with anti-cancer activity of methanolic extracts from the leaf of A. marmelos.
 Methods: Standard methods for antioxidant potential in terms of DPPH and nitric oxide scavenging assay, and anticancer activity in vitro method (Cytotoxicity/MTT assay and % of cell viability) by using MCF7 cell line.
 Results: Results of antioxidant efficacy revealed that the IC50 value for DPPH and nitric oxide scavenging assay was considered to be 62.032%, and 20.69% respectively. The methanolic extract of A. marmelos was found to possess enhanced anticancer potential against MCF7 cells. Cytotoxicity activity of MCF7 cells, when treated with methanolic extract of A. marmelos, was found to be 43.42% at 25µg, 52.31% at 50µg, 56.31% at 75µg, 58.38% at 100µg, 62.25% at 125µg. The IC50 value was found as 49.36µg. Toxicity was significantly increased with increased concentration and viability significantly decreased with the increased concentration of methanolic extract of leaf from A. marmelos for MCF7 cell when compared to cyclophosphamide.
 Conclusion: From the studies, it was postulated that methanolic extract of leaf from A. marmeloshas significant chemopreventive activity. These specific identities will be useful for the identification and authentication of raw drug.
Highlights
Cancer is an important public health problem, with significant associated global mortality, disability, and the contribution of risk factors
The antioxidant and anticancer activity of leaves from A. marmelos has never been investigated. This present communication attempt had been evaluated the effectiveness of antioxidant activity (DPPH and nitric oxide radical scavenging assay) along with in vitro anticancer activities (% of cell viability and cytotoxicity/MTT assay by using MCF7 cell) in methanolic extracts from leaf of A. marmelos
The principle of the DPPH method is based on the reduction of DPPH in the presence of a hydrogen donating antioxidant owing to the formation of diphenylpicrylhydrozine
Summary
Cancer is an important public health problem, with significant associated global mortality, disability, and the contribution of risk factors. It is the second leading cause of death in developed countries [1]. A. marmelos is a perennial tree, wild in Sub Himalaya tract, central and South India. This plant is usually called as bael in hindi, vilvam in tamil and bilva in sanskrit. The antioxidant and anticancer activity of leaves from A. marmelos has never been investigated. This present communication attempt had been evaluated the effectiveness of antioxidant activity (DPPH and nitric oxide radical scavenging assay) along with in vitro anticancer activities (% of cell viability and cytotoxicity/MTT assay by using MCF7 cell) in methanolic extracts from leaf of A. marmelos
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