Abstract
ABSTRACT The present study was conducted to evaluate the antioxidant and antimicrobial activities of methanol extracts of Anthemis fumariifolia and Anthemis cretica subsp. argaea, which are commonly used in Turkish folk medicine. The total phenolic and total flavonoid contents in the extracts were determined. The antioxidant activities of the extracts were measured employing three methods, namely phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazyl and β-carotene-linoleic acid assays. The A. fumariifolia and A. cretica subsp. argaea extracts behaved as strong free radical scavengers with IC50 = 22.65 and 21.43 µg/mL, respectively. In the β-carotene-linoleic acid system, A. fumariifolia and A. cretica subsp. argaea extracts exhibited 55.41 and 59.10% inhibition against linoleic acid oxidation, respectively. Antimicrobial activity was assessed by the agar diffusion method against 13 bacteria and two yeasts. Test results showed that the extracts had great potential of antibacterial activity against many bacteria tested. However, they had no inhibitory effect on Candida albicans and Saccharomyces cerevisiae. PRACTICAL APPLICATIONS A. fumariifolia Boiss. and A. cretica L. subsp. argaea (Boiss. & Ball.) Gierson are two species endemic to Turkey. The Anthemis species are known to possess various biological activities and are extensively used as tea in Turkish folk medicine for the treatment of many disorders. The results presented here suggest that the methanol extracts of A. fumariifolia and A. cretica subsp. argaea may possess antioxidant and antimicrobial properties, and can therefore be used as natural agents in the food and pharmaceutical industries.
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