Evaluation of Antioxidant and Antimicrobial Activities of Potentilla recta L.

Abstract

In this study, the antioxidant activity and effectiveness against pathogenic microorganisms in the skin were investigated to evaluate the wound healing mechanisms of Potentilla recta L. (Rosaceae), which is traditionally used in wound treatment. The antioxidant activity was determined using 1,1-Diphenyl-2-picrylhydrazyl (DPPH•) and2,2-Azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) radical scavenging activities, inhibition of β-carotene bleaching, protection of 2-deoxyribose and bovine brain-derived phospholipids against hydroxyl radical-mediated degradation assay, at different concentrations ranging from 0.001 to 2 mg mL-1. By the microdilution method, antimicrobial activity was evaluated on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans. Besides, viability enhancing effects on murine fibroblast cells (L929) were determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The key parameters for the extract included the following: DPPH• IC50: 0.19 mg mL-1, TEAC: 0.76 mmol L-1 Trolox, reduction: 0.87 mmol g-1 AsscE, and protection of lipid peroxidation IC50: 0.07 mg mL-1. A strong effect on S. epidermidis was observed with 79% inhibition at a concentration of 125 mg mL-1, but did not show toxicity to L929 cells below 250 mg mL-1 concentration. The results proved that the potent antioxidant activity combined with the antimicrobial activity supports traditional use.