Abstract
Purpose: To evaluate the antioxidant and antifungal activities of polyphenol-rich extracts of the dried fruit pulp of Garcinia pedunculata (GP) and Garcinia morella (GM) to determine their traditional claims of therapeutic activity against certain diseases.Methods: Analysis of total phenolic (TP) and flavonoid (TF) contents of the extracts were performed by Folin-Ciocalteau and Arvouet-Grand methods. The antioxidant activity of the extracts was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) free radical scavenging activity, reducing power and in vitro lipid peroxidation (LPO). Antifungal activity was evaluated by agar-well diffusion method while mineral content was evaluated by atomic absorption spectrophotometry (AAS).Results: Significant amounts of TP (5.87 ± 0.06 and 5.46 ± 0.02 mg catechin eqivalents/g) and TF (5.61 ± 0.16 and 3.69 ± 0.04 mg quercetin equivalents/g) were found in the cold water (CW) extracts of GP and GM, respectively, along with DPPH free radical scavenging activity (50 % inhibitory concentration (IC50) = 3.53 ± 0.04 and 1 ± 0.03μg/mL) and H2O2-radical scavenging activity (IC50 = 1.4 ± 0.02 and 1.44 ± 0.01 μg/mL). Results indicated that the CW extracts of GP and GM were potent reducing agent than the HW extracts. CW extract of both species prevented in vitro LPO (IC50= 42 ± 0.01 and 30.36 ± 0.03 μg/mL) significantly. The antifungal activity of GP and GM extracts against some human dermatophytes was high. High concentrations of K and Fe were found in the extracts.Conclusion: GP and GM extracts have great potential as a source for useful antioxidant and antifungal agents.Keywords: Antioxidant, Phenolic, Flavonoid, Lipid peroxidation, Antifungal, Dermatophytes
Highlights
Reactive oxygen species (ROS) such as superoxide anion (O-2), perhydroxy radical (HOO), hydroxyl radical (HO-) and nitric oxide (NO) radical are generated during the process of cellular oxidation
It was revealed that the total phenolic content (TPC) in cold water (CW) extracts was higher than that of hot water (HW) extracts
In the case of DPPH, the IC50 values of the CW extracts were much lower (3.53 ± 0.04 and 1 ± 0.03 μg/mL) than than the standard ascorbic acid (5.56 ± 0.03 μg/mL) and it indicated that the Garcinia pedunculata (GP) and Garcinia morella (GM) contained high antioxidant activity
Summary
Reactive oxygen species (ROS) such as superoxide anion (O-2), perhydroxy radical (HOO), hydroxyl radical (HO-) and nitric oxide (NO) radical are generated during the process of cellular oxidation. These electrically charged radicals are highly unstable, reactive in nature and attack proteins, nucleic acid, mitochondria and enzymes in biological systems resulting damage in the cell. High levels of reactive free radicals and oxygen species create oxidative stress, which leads to detrimental effects, including lipid peroxidation (LPO) of cellular membranes, alteration of lipid–protein interaction, enzyme inactivation, DNA breakage, and eventually the promotion of mutations that initiate tumor progression [1,2]. It is important to evaluate the consumption of those fruits and vegetables which have been traditionally claimed for having therapeutic values
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
