Evaluation of antioxidant activity, quantitative estimation of phenols and flavonoids in different parts of Aegle marmelos

Abstract

In existing study, we carried out an efficient record of the comparative antioxidant activity in methanolic extract of the selected parts (leaves, root and stem bark) of Aegle marmelos. Total content of phenol and flavonoid was quantitatively estimated in different parts of A. marmelos. The total phenolic contents varied from 9.8367 ± 0.0235 to 1.7281 ± 0.049 mg g −1. Total flavonoid contents were between 8.248 ± 0.029 and 1.087 ± 0.002 mg g −1. Free radical scavenging activity of different extracts was evaluated by using DPPH (1,1-diphenyl-2-picryl hydrazyl) method. The highest free radical scavenging effect was observed in leaves with IC 50 = 2.096 μg ml −1. The effectiveness of radical scavenging activity of leaves extract was about 10 times greater than reference antioxidant butylated hydroxy toluene (BHT). The greater amount of phenolic compounds leads to more powerful radical scavenging effect as shown by methanolic extract of A. marmelos leaves.