Abstract
Objective: To investigate the antioxidant activity of magnolol in Saccharomyces cerevisiae (S. cerevisiae) model.Methods: Antioxidant activity of magnolol was demonstrated in S. cerevisiae using spot assay, colony forming unit (CFU) assay, detection of reactive oxygen species (ROS) by fluorescent microscopy and spectrofluorometer, lipid peroxidation (LPO) and catalase (CAT) assay.Results: A dose-dependent increase in sensitivity of S. cerevisiae was observed with increase in hydrogen peroxide (H2O2) concentration. At 1.5 mmol concentration of H2O2, we observed 50 % of cell survivability in CFU and spot assay results. The sensitivity of S. cerevisiae to H2O2 was protected by magnolol treatment. In spot assay, magnolol pre-treatment showed the similar growth pattern as that of control and in CFU assay 75 % of survivability was observed. Fluorescence microscopic images and fluorescence intensity levels using 2´, 7´-dichlorodihydrofluoresceindiacetate (H2DCFDA) showed less number of fluorescence cells and 1.2 fold decrease in fluorescence intensity in magnolol pre-treated cells. The cellular protection in the cells pre–treated with magnolol followed by H2O2 was correlated with the decreased LPO and increase in CAT activity was recorded.Conclusion: The result of the present study demonstrates that magnolol protects S. cerevisiae cells from H2O2 induced oxidant mediated cell death.
Highlights
reactive oxygen species (ROS) mediated oxidative stress plays a significant role in human health and disease management
In spite of extensive studies on the antioxidant property of magnolol in vitro, the molecular mechanism how it works in vivo remains unclear we investigate the antioxidant activity of magnolol in S. cerevisiae model
Optimization of H2O2 concentration to study the antioxidant activity of magnolol
Summary
ROS mediated oxidative stress plays a significant role in human health and disease management. Plant phytochemicals possess antioxidant properties play a significant role as therapeutic agent to overcome the oxidative stress induced by ROS. Plant derived phytochemicals such as polyphenols, alkaloids flavanoids, terpenoids, and saponins are gaining much importance in many diseases such as cardiovascular diseases, neurodegenerative diseases and cancers.[2]. Polyphenols are the most abundant antioxidant phytochemical present in fruits, vegetables, red wine and legumes, coffee, and tea These polyphenols act either by activating the antioxidant enzymes or by scavenging the free radicals. Magnolol is an active polyphenolic compound isolated from Magnolia officinalis (M. officinalis) a Chinese plant[3] It possess various medicinal properties includes antioxidant, anticancer, antiinflammatory and antimicrobial without any toxicity[4]. We demonstrated antioxidant activity of magnolol in S. cerevisiae model
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Pharmacy and Pharmaceutical Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
