Abstract
Objective : To obtain a systematic record of the relative antioxidant activity of flavonoid and phenolic contents of Luffa echinata Roxb fruit and Nyctanthus arbor-tristis leaves extracts. Methods : Phenolic and flavonoid content were determined by using gallic acid and quercetin as standard, antioxidant properties were evaluated by the methods, namely the Trolox equivalent antioxidant capacity assay (TEAC), Oxygen radical absorbance capacity (ORAC) Assay and DPPH free radical scavenging activity assay. Results : Total phenolic content were found 76.34±0.44 and 45.53±0.65 mg/g, while flavonoid contents were found 65.98±0.83 and 34.92±0.76 mg/g in Luffa echinata Roxb and Nyctanthus arbor-tristis respectively. In the Trolox equivalent antioxidant capacity assay, TEAC value were found 0.34 mmole/g and 0.28 mmole/g, while in Oxygen radical absorbance capacity assay, ORAC value was found 253.7m moles TE # / g and 221.6 m moles TE # / g, where as DPPH free radical scavenging activity assay showed IC 50 value 188±0.87 and 176±0.68 for Luffa echinata Roxb and Nyctanthus arbor-tristis extracts . Conclusion : The present studies suggest that both the plants have moderate to potent antioxidant activity.
Highlights
Free radicals contribute to more than one hundred disorders in humans including atherosclerosis, arthritis, ischemia, injury of many tissues, central nervous system injury, gastritis, cancer and AIDS
2.1 Chemicals 1, 1-Diphenyl -2-picryl hydrazyl (DPPH) and quercetin were purchased from Sigma chemicals Co, Vadodara, Gujarat
Phenolic compounds are a class of antioxidant agents, which act as free radical scavengers[9].The Table No.[2] show the contents of total flavonoid contents in terms of quercetin equivalent (The std curve equation: y =0.00319X + 0.08995, r2 = 0.985) (Figure No.1) and phenols that were measured by Folin Ciocalteu reagent in terms of Gallic acid equivalent (The std. curve equation: y = 0.00174X +0.01239, r2 =0.991) (Figure No.2)
Summary
Free radicals contribute to more than one hundred disorders in humans including atherosclerosis, arthritis, ischemia, injury of many tissues, central nervous system injury, gastritis, cancer and AIDS. Catalase and hydroperoxidase enzymes convert hydrogen peroxide and hydro peroxides to non radical forms and functions as natural antioxidants in human body. Free radicals cause depletion of immune system antioxidants, causes change in gene expression and induce abnormal proteins. Available synthetic antioxidants like butylated hydroxyl anisole (BHA), butylated hydroxyl toluene (BHT), tertiary butylated hydroquinones and gallic acid esters have been suspected to cause or prompt negative health effects[1]. It has been suggested that natural antioxidants are more safe and healthy than synthetic antioxidants[2].
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