Abstract
Antioxidant play an important role to protect damage caused oxidative stress (OS).Chlorophyll having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties of methanolic solution from Super Chlorophyll.
 Objective: The main objective of the study was to evaluate the antioxidant activity of the chlorophyll in different validated in vitro models.
 Methods: The antioxidant activities of methanolic solution were evaluated by in vitro standard method using UV-Spectrophotometer. The antioxidant activity were determined bt total antioxidant capacity, DPPH (1-1-diphennyl-2-picrylhydrzine) radical scavenging assay, Nitric Oxide scavenging assay and Hydrogen Peroxide scavenging assay methods.
 Result: The solution of Chlorophyll was studied for antioxidant potential. Ascorbic acid was used as standard. This method is simple and activity of the solution is reported in term of IC50 value. Antioxidant properties depend on the IC50 value. Lesser the IC50 value, more is the antioxidant activity. Chlorophyll showed good antioxidant activity.
 Conclusion: In case of in vitro antioxidant activity, Chlorophyll scavenged DPPH, hydrogen peroxide, nitric oxide radicals significantly, showed IC50 values near to standard ascorbic acid, thus proving to have good antioxidant potential.
 Keywords: Oxidative stress, DPPH, Antioxidant, Super chlorophyll.
Highlights
Antioxidant: A substance that reduces damage due to oxygen, such as that caused by free radicals
Antioxidant properties depend on the IC50 value
The data obtained in the present study suggests that the alcoholic extract of C. siamea flowers have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage in the liver.[30]
Summary
Antioxidant: A substance that reduces damage due to oxygen, such as that caused by free radicals. Well-known antioxidants include enzymes and other substances, such as vitamin C, vitamin E, and beta carotene, which are capable of counteracting the damaging effects of oxidation. Antioxidants may possibly reduce the risks of cancer. Antioxidants clearly slow the progression of age-related macular degeneration.[1]Living cells generate free radicals and other reactive oxygen species (ROS) by-products as a result of physiological and biochemical processes. Antioxidant dietary supplements have been not shown to improve health in humans, or to be effective at preventing disease.[2] Supplements of beta-carotene, vitamin A, and vitamin E have no positive effect on mortality rate[3][4] or cancer risk. Supplements of beta-carotene, vitamin A, and vitamin E have no positive effect on mortality rate[3][4] or cancer risk. [5][6] supplementation with selenium or vitamin E do not reduce the risk of cardiovascular disease.[7][8]
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