Abstract
The aim of this study was to investigate the antioxidant potential of ampelopsin (APS) by using various methods in vitro, as well as to determine effects of APS on LPS-induced oxidative stress in piglets. The results showed that APS exhibited excellent free radical scavenging by DPPH, ABTS, O2•−, H2O2 and ferric reducing antioxidant power. Ampelopsin also protected pig erythrocytes against AAPH-induced apoptosis and hemolysis, decreased total superoxide dismutase activity, and increased lipid peroxidation. Furthermore the results demonstrated that APS enhanced the total antioxidant capacity and decreased the malondialdehyde and protein carbonyl contents in LPS-treated piglets. The results of the present investigation suggest that APS possesses a strong antioxidant activity and alleviates LPS-induced oxidative stress, possibly due to its ability to prevent reactive oxygen species.
Highlights
Ampelopsin (3,5,7,39,49,59-hexahydroxy-2,3-dihydroflavonol, APS), is the main bioactive component and most common flavonoid in dry tender stems and leaves
We evaluated the antioxidative effects of APS on the oxidative stress of pig erythrocytes induced with 2,29-azobis-2-amidinopropane dihydrochloride (AAPH), a free radical generator, to test the markers of oxidative stress, including hemolysis, lipid peroxidation, superoxide dismutase (SOD) enzyme activity, and phosphatidylserine exposure on the cell surface, to investigate the protective effect of APS on early cellular apoptotic responses to oxidative stress
Antioxidants have become of interest to biologists and clinicians because they can protect the body from oxidative damage
Summary
Ampelopsin (3,5,7,39,49,59-hexahydroxy-2,3-dihydroflavonol, APS), is the main bioactive component and most common flavonoid (about 20–30% w/w) in dry tender stems and leaves. APS comprises more than 40% of the flavonoid content in the cataphyll of the plant Ampelopsis grossedentata (Hand-Mazz) W.T. Wang. It is known as vine tea, and has been a health beverage in the south of China for thousands of years. Ampelopsin plays a wide variety of health roles, including antiinflammatory, anti-hypertension, cough relieving, antioxidant, hepatoprotective, antimicrobial, and anticarcinogenic actions [1,2]. To the best of our knowledge, there are so far no systematic reports relating to the antioxidant capacity of APS using both in vivo and in vitro assays
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