Evaluation of Antioxidant activities and total phenolic content of hydro-ethanol extract from Phlomis bovei De Noé areal parts

Abstract

The plants of the genus Phlomis are native to Turkey, North Africa, Europe and Asia. Phlomis bovei De Noé (Lamiaceae) is a rare Algerian endemic plant, commonly known as Kayat El Adjarah. The objective of this study is to quantify the polyphenol content and to evaluate the antioxidant activities of the ethanolic extract (EthE) of Phlomis bovei De Noé. The plant extract was prepared by macerating 100 g of ground material in 1000 ml of 85 % ethanol for 72 h, then the filtrate was evaporated using a rotary evaporator at a temperature of 45C and the filtrate was dried. The total content in polyphenols was determined using Folin Ciocalteu method. Flavonoid content in extracts was determined using Aluminum trichloride assay and the total content of tannins was determined according to Bate Smith method. The antioxidant activity was investigated in vitro by the DPPH (2, 2-diphenyl-1-picryl-hydrazyl), ABTS and iron chelating assays. The results indicate that the ethanol extract was rich in polyphenols and has an important capacity of scavenging the DPPH and ABTS free radical with an IC50 of 0.05 and 0.018 mg/ml, respectively. However, in the ferrous ions chelating ability test, PBEE extract showed moderate chelating activity (IC50 = 1.59 ± 0.05 mg/ml). To conclude, the obtained results may contribute to add possible scientific data to use Phlomis bovei industrially as herbal medicine and as supplementary sources for natural antioxidant drugs in food.
 Keywords: antioxidant activity, ethanol extract, Phlomis bovei De Noé, polyphenol content.