Evaluation of antimicrobial photodynamic therapy with toluidine blue against Enterococcus faecalis: Laser vs LED.

Abstract

This study aimed to compare the antibacterial effect of antimicrobial photodynamic therapy (aPDT) by use of light emitting diode (LED) and diode laser light sources with toluidine blue (TBO) photosensitizer on Enterococcus faecalis (E. faecalis) biofilm in root canals of extracted single-canal human teeth. Eighty-five sound human single-canal teeth were chosen for this study and standardized to have 15 mm of root length. The root canals were prepared with ProTaper rotary files and inoculated and incubated with E. faecalis for one week. Samples were divided into five experimental (n = 15) groups of PDT with TBO/LED, TBO/diode laser, LED, TBO and diode laser and one negative (NaOCl) and one positive (no treatment) control group. Dentin chip samples were collected from inside the canals using size 40 hedstrom file. The number of colony forming units (CFUs) in each group was calculated. Irrespective of the light source used for activation of photosensitizer (diode or LED), PDT was significantly more effective than other experimental groups (P < 0.001). No significant difference was noted between aPDT with diode laser or LED (P > 0.05). No significant difference was noted in colony count among other groups (LED, TBO, diode; P > 0.05). The results showed that aPDT significantly decreased residual bacteria in the canal. Thus, it may be used as an adjunct for root canal disinfection. Both diode and LED are suitable light sources for this purpose and can be used alternatively.