Evaluation of antimicrobial efficacy of three calcium silicate-based materials using tube dilution: An in vitro study

Abstract

Periapical pathosis is considered as a persistent microbial infection throughout the treatment period of root canal procedures. Recently the prevalence of endodontic failures is huge and this primarily accounts for the periapical pathogens; mainly Enterococcus faecalis and Candida albicans. Thus, with the advent of newer materials; identification of the most appropriate sealing material with highest antimicrobial efficacy is essential for endodontic success. To evaluate and compare the antimicrobial efficacy of MTA Angelus, Biodentine and Theracal LC against Enterococcus faecalis and Candida albicans. An in-vitro study was conducted using three silicate-based cements; MTA Angelus, Biodentine and Theracal LC. Their antimicrobial efficacy was evaluated using Tube dilution method and their Minimum inhibitory concentration (MIC) and Bacterial counts (CFU’s) were assessed and compared. Statistical analysis was done using Paired t-test and ANOVA at 95% confidence interval (p value <0.05).The MIC was highest and CFU’s were lowest with Biodentine in comparison with other two materials.There were statistically significant differences noted between Biodentine and MTA angelus: Our study results conclude that Biodentine was found to be more anti-bacterial as compared to MTA angelus and theracal LC.