Abstract
Purpose: This study aimed to evaluate the antimicrobial efficacy of nano chitosan and chlorohexidine versus sodium hypochlorite as final rinse against Enterococcus faecalis. Methods: Forty single rooted teeth were selected. All root canals were prepared manually to #50 file. Finally, the teeth were sterilized by gamma rays. Each root canal was inoculated with 0.5 ml of E. faecalis. Specimens were divided randomly into 4 main groups (10 specimens each) according to the irrigating solutions. Group 1: nano chitosan 0.2%, group 2: Chlorhexidine 2%, group 3: alternating solution of nano chitosan 0.2% & Chlorhexidine 2%, and group 4: Sodium hypochlorite 3%. The mean of bacterial count before and after final irrigation by the tested solutions were measured. Results: The highest mean difference value of bacterial count was found in 3% sodium hypochlorite, followed by alternating solution of 0.2% nano chitosan & 2% chlorhexidine, then 2% chlorhexidine, and 0.2% nano chitosan came lastly. However, there was no statistically significant difference among the tested groups. Conclusions: alternating solution of 0.2% nano chitosan & 2% chlorhexidine when used as antibacterial irrigant gives promising results and can be used as an alternative to NaOCl solution.
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