Evaluation of Antimicrobial Efficacy of Murraya Koenigii Plant Leaf Extract Against E. coli Isolated from Different Sources

Abstract

The current study was carried out to evaluate the anti-microbial efficacy of the leaf extract Murraya koenigii plant against E. coli isolated from clinical and various other sources like water, soil, skin, floor, juice, basin, incubator and mobile phone. The anti-microbial test showed that the ethanolic extract was more potent than aqueous extract of Murraya koenigii leaves against E. coli. The sensitivity of the bacterial isolates from each sample having lowest potential against the extract was examined against four different antibiotics. It was observed that all isolate showed complete resistance to Amoxicillin and were sensitive to Ciprofloxacin, Chloramphenicol and Gentamycin. Qualitative analysis of active compounds in extract of Murraya koenigii leaves was done using different chemical reagents. The result showed the presence of phenols, flavonoids, terpenoids, tannins and quinones. The Total Antioxidant Capacity was calculated to be 40.42%. UV-VIS spectrophotometric analysis of extract of Murraya koenigii leaves in different solvents like distilled water, ethanol and methanol was done. The analysis exhibited peaks at wavelength 420 and 500 nm for methanol and 500nm for distilled water and ethanol.