Abstract
Extreme environments Morrope and Bayovar Salt lagoons, several ecosystems and microhabitats remain unexplored, and little is known about the diversity of Actinobacteria. We suggest that the endemic bacteria present in this extreme environment is a source of active molecules with anticancer, antimicrobial, and antiparasitic properties. Using phenotypic and genotypic characterization techniques, including 16S rRNA sequencing, we identified these bacteria as members of the genera Streptomyces, Pseudonocardia, Staphylococcus, Bacillus, and Pseudomonas. Actinobacteria strains were found predominantly. Phylogenetic analysis revealed 13 Actinobacteria clusters of Streptomyces, the main genus. Three Streptomycetes, strains MW562814, MW562805, and MW562807 showed antiproliferative activities against three tumor cell lines: U251 glioma, MCF7 breast, and NCI-H460 lung (non-small cell type); and antibacterial activity against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, and the multidrug resistant Acinetobacter baumannii AC-972. The antiproliferative activities (measured as total growth inhibition [TGI]) of Streptomyces sp. MW562807 were 0.57 μg/mL, for 0.61 μg/mL, and 0.80 μg/mL for glioma, lung non-small cell type, and breast cancer cell lines, respectively; the methanolic fraction of the crude extract showed a better antiproliferative activity and could inhibit the growth of (U251 (TGI = 38.3 μg/mL), OVCAR-03 (TGI = 62.1 μg/mL), and K562 (TGI = 81.5 μg/mL)) of nine tumor cells types and one nontumor cell type. Extreme enviroments, such as the Morrope and Bayovar Salt saloons are promising sources of new bacteria, whose compounds may be useful for treating various infectious diseases or even some types of cancer.
Highlights
Several regions in Peru have extreme environments, such as the salt marshes located on the coast, center, and south of the country
We performed a phylogenetic analysis of a collection of bacterial isolates from the saline lagoons of northwestern Peru. We explored their potential as producers of secondary metabolites with antimicrobial and antiproliferative activities. we have revealed that the Actinobacteria existing in these extreme environmental conditions have diverse characteristics and could comprise new species that produce novel and biologically active compounds
13 bacterial isolates from the saline lagoons of northwestern Peru (Morrope and Bayovar) cultivars were identified after the 16S rRNA gene sequencing (Fig 2)
Summary
Several regions in Peru have extreme environments, such as the salt marshes located on the coast, center, and south of the country. Halophilic and halotolerant strains of Actinobacteria show heterogeneous physiological characteristics for different genera because these bacteria can synthesize secondary metabolites to cope with the high salinity and extreme temperature conditions of their environments [7,8,9] These extreme conditions favor the development of metabolic competitiveness to the production enzyme, which can bacterial population adapt to high salinity [10]. These microorganisms can perform essential processes, such as carbon cycle, metal transfer, and the removal of organic pollutants at higher trophic levels [11, 12]. Information on substances isolated from microorganisms inhabiting saline environments is scarce
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