Evaluation of Antimicrobial and Antifungal Effects of Ag and Mg Dual-Doped Zinc Oxide Nanoparticles against Staphylococcus Epidermidis, Pseudomonas Aeruginosa, and Candida Albicans

Abstract

Background: Microbial infectious diseases are one of the serious health problems that have attracted the attention of the public as a threat to human health all over the world. Zinc oxide nanoparticles due to their various properties such as antibacterial, antifungal, and anticancer are of great interest. Methods: The antimicrobial activity of synthesized un-doped and silver and magnesium-doped zinc oxide nanoparticles with Salvadora persica extract against different strains of Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans was investigated through two methods of broth diffusion and the minimum microbial inhibitory concentration (MIC). Results: According to results, MIC of un-doped and silver and magnesium-doped zinc oxide nanoparticles showed 160, 80, 40, and 40 µg/mL on Staphylococcus epidermidis, and 80, 40, 40, and 40 µg/mL on Staphylococcus aureus. These values on Pseudomonas aeruginosa were obtained ˃5120, 640, 320, and 320 µg/mL. Conclusion: The results of the broth dilution method on Staphylococcus epidermidis and Staphylococcus aureus showed the presence of silver has a very good inhibition effect on these bacteria. In Pseudomonas aeruginosa and Candida albicans fungus, it has been observed that by increasing the amount of silver and magnesium doping, the inhibitory rate of nanoparticles on Gram-negative bacteria and fungi has increased.