Abstract

Aims: The objective of present study was to investigate the chemical analysis biological activates of commiphora myrrh the chemical analysis of myrrh was analyzed several ways.
 Study Design: Where wet digestion was used to estimate the concentrations of a number of chemical elements in it, which are of great importance to humans and are also attributed to many of its medicinal uses.
 Place and Duration of Study: Clinical isolates from wound infections obtained from laboratory of Marjan Hospital Hilla city Iraq during period Fed. 2020 which include (four E. coli and 4 of S. aureus, and 4 pseudomonas aeruginosa ). All clinical isolates were classified and by laboratory of marjan hospital: Hilla city Iraq.
 Methodology: In order to know the nature of the groups present in it, in addition to the quality of the organic materials, FTIR analysis and G.C analysis were used by using the ethanolic extract, where some of the organic materials within their compositions in identified.
 Results: The antimicrobial potential of ethanolic extracts of myrrh were studied against many standard strains of gram positive and gram negative bacteria and (12) clinical isolated from patients with wound infections obtained from the bacteriology section of the clinical microbiology laboratory of marjan hospital Hill city/Iraq during period Feb. 2020 to Nov. 2020. The clinical isolates include (4) isolates of staphylococcus aureus, (4) isolates of E.coli and (4) isolates of pseudomonas aeruginosa, and it was confirmed using the usual methods of diagnosis. The broth dilution method was used for determination of the MICs of (minimal inhibitory concentration) of myrrh extract against pathogens under study. Six concentration (80, 60, 30, 12, 6, 3 mg/mL) of myrrh extracts were tested.
 Conclusion: The result revealed that the highest activity was against S. Aureus at concentration (80, 60, 30 mg/mL) which showed completely inhibition of the growth (100%). While the gram-negative bacteria E.coli and P. auroginosa the concentration (80 – 60 mg/mL) showed 100% inhibition in contrast the concentration (12, 6, 3 mg/mL) showed no activity of myrrh extract against all pathogens under study. The result indicates that myrrh is an antibacterial agent that can be used in the future by making appropriate doses.

Highlights

  • Myrrh is one of the important and widely used medicinal plants, as it was used by the ancient Egyptians as medicine [1]

  • Twelve clinical isolates was obtained from marjan hospital laboratory to ensure their purity and identification we started with microscopic examination using gram stain technique (Syed Rizwan et al 2017)

  • Four isolates of S. aurous cultured on nutrient agar, showed golden yellow colonies on mannitol salt agar it changed the color of medium from red to yellow with gram stain. under microscope appeared as gram positive cocci arranged in grape like clusters knees applications ensured the identification of four isolates of clinical S

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Summary

INTRODUCTION

Myrrh is one of the important and widely used medicinal plants, as it was used by the ancient Egyptians as medicine [1]. It belong to kingdom: Plantae ,division : magnoliophyte class : magnoliopsida :order: Spindale's ,family burseraceous :genus commphorra [4]. It is a small tree with a short true myrrh plant produced by C. It tends not to dissolve very well in water [12] Myrrh contains a 2-8% volatile oil (myrrhol), 23-40% resin (myrrhin), 40-60% gum, and a bitter principle10-25% [13]. The main Chemical compounds of Myrrh [15]

MATERIALS AND METHODS
Preparation Test Organisms
Determination of MIC
Chemical Analysis
Clinical Isolates
MIC for Clinical Isolates
Chemical Analytics
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