Abstract
The isolated compounds from the petroleum spirit, chloroform and methanol crude leaf extracts of the white specie of the leaf of the Sesamum indicum used by traditional medicinal practitioners for the management of infecticious diseases were investigated for in vitro antimicrobial activity against some organisms. The various extracts afforded compounds which had significant antimicrobial activities. The antimicrobial screening showed that all the pure isolates from the different solvent extracts were active against the organisms, Staphylococcus aureus, Streptococcus pyogenes, Salmonella typhi, Candida albicans, Candida krusei, and Candida tropicalis at various MIC’s and (MBC/MFC)’s. The pure isolates from methanol and ethyl acetate fractions were all inactive against Bacillus cereus, Corynebacterium ulcerans, and Escherichia coli. Only the pure isolates from petroleum spirit and chloroform fractions showed activity against Bacillus cereus, Corynebacterium ulcerans, and Escherichia coli at an MIC of 0.5mg/ml and MCB/MFC of 1mg/ml. Chromatographic techniques such as analytical TLC, Purification by dry vacuum liquid chromatography (DVLC) and Preparative TLC were used. ©JASEMKeywords: Anti-microbial, Benue, Pathogenic micro-organisms, Sesamum indicum leaves, Dry vacuum liquid chromatography (DVLC).
Highlights
Throughout history, natural products from plants have played major sustaining roles in the lives of humans, especially as food sources and for medicinal purposes
The compound (2) from petroleum spirit fraction of Sesamum indicum inhibited the growth of Staphylococcus aureus, Streptococcus pyogenes, Salmonella typhi, Candida albicans, Candida krusei, Bacillus cereus, Corynebacterium ulcerans, Escherichia coli and Candida tropicalis at a concentration of 0.5mg/l, with the corresponding MBC at 2mg/l for Streptococcus pyogenes, Salmonella typhi, Candida albicans, Candida krusei, Corynebacterium ulcerans, Candida albicans, expect for Staphylococcus aureus, Bacillus cereus, and Escherichia coli which had their MBC at 1mg/l
The compound (3) a reddish oil from petroleum spirit MBC at 2mg/l for, Staphylococcus aureus, fraction of Sesamum indicum inhibited the growth of Salmonella typhi, Candida albicans, Candida krusei, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Candida albicans, expect for Salmonella typhi, Candida albicans, Candida krusei, Streptococcus pyogenes, and Bacillus cereus, which
Summary
Throughout history, natural products from plants have played major sustaining roles in the lives of humans, especially as food sources and for medicinal purposes. Antimicrobial Screening: Zone Of Inhibition: The antimicrobial activities of the various extracts from the plant Sesamum indicum were determined using some pathogenic microorganisms.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Applied Sciences and Environmental Management
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
