Abstract
IntroductionIsolated bioactive components of plants or their raw extract are utilized as complementary or alternate remedy in copious illnesses. The current research was aimed at assessing the activity of aloin A isolated from Aloe barbadensis Miller and its formulated ointment against six (6) selected clinical isolates.MethodsThe column chromatography was utilized in isolating aloin A from chloroform/methanol solvent polarity. The characterization of the isolated compound was performed by spectroscopy techniques corresponding to UV, IR, 1H- and 13C-NMR spectroscopy. It was formulated as ointment using polyethylene glycol (PEG) and both the ointment and the isolated compound were probed for in vitro antimicrobial activity.ResultsAloin A has been isolated from chloroform/methanol solvent mixture. The structure has been explicated as (10S)-10-β-d-glucopyranosyl-1,8-dihydroxy-3-(hydroxymethyl)-9(10H)-anthracenone(1S)-1,5-anhydro-1-[(9S)-4,5-dihydroxy-2-(hydroxymethyl)-10-oxo-9,10-dihydro-9-anthracenyl]-d-glucitol. The minimum inhibitory concentration (MIC) of the isolated aloin A on the pathogens ranged from 2.5 to 5.0 mg/ml and 0.32 to 5.0 mg/ml for both aloin A and the formulated ointment respectively. It was further revealed that the activity of aloin A showed dose dependence against all the test microorganisms. There was no significant difference in the activity of the drug against K. pneumoniae, S. aureus, E. coli, C. albicans and T. flavus (P > 0.05) when the concentration was raised from 2.5 to 5 mg/ml, however, there was significant difference (P ˂ 0.05) in activity against P. aeruginosa. The formulated ointment exhibited dose dependent activity against all test microorganisms. At low concentrations, the ointment showed no significant difference in diameter zone of inhibition against all test microorganisms (P > 0.05) except P. aeruginosa which exhibited a highly significant difference (P < 0.05).ConclusionBoth the isolated aloin A and its formulated ointment demonstrated substantial inhibition of growth of the pathogenic strains. These findings sturdily suggest that aloin A is a nascent drug that could be explored as skin and wound transmittable agent.
Highlights
Isolated bioactive components of plants or their raw extract are utilized as complementary or alter‐ nate remedy in copious illnesses
S. aureus is frequently described as being responsible for a variety of human and animal diseases and its epidemiological significance is mainly due to their ability of becoming highly resistant to common antimicrobials [3,4,5] and to a lesser degree to some of the major types of antibiotics [6, 7]
Preparation of plant crude extracts Aloe barbadensis Miller leaves were washed with distilled water and dried under sunshade for 4 weeks to ensure the leaves were devoid of moisture
Summary
Isolated bioactive components of plants or their raw extract are utilized as complementary or alter‐ nate remedy in copious illnesses. One significant problem in human health is the less efficiency of commercial antibiotics against several pathogenic bacterial and fungal isolates. S. aureus is frequently described as being responsible for a variety of human and animal diseases and its epidemiological significance is mainly due to their ability of becoming highly resistant to common antimicrobials [3,4,5] and to a lesser degree to some of the major types of antibiotics [6, 7]. Candida albicans is a member of the normal human microbiome. It is an opportunistic fungal pathogen associated with infections such as thrush, vaginal yeast infections and diaper rash [8]. The mortality rate of candida infections is reported to be 40% [9, 10]
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