Evaluation of Antidiabetic Activity of Vitis pedata in Alloxan Induced Diabetic Rats

Abstract

The primary focus of the research was to evaluate the antidiabetic activity of the ethanolic and aqueous extract of Vitis pedata in alloxan induced rats. The entire study was divided into two phases: Phase 1 and phase 2. The phase 1 is initiated with the collection and authentication of the plant, followed by extraction with ethanol and water and finally screening of the phytochemical constituents of the extracts. The phase 2 is more inclined on the therapeutic effect of the plant extracts on experimentally induced diabetic rats. Several parameters such as estimating the body weight and liver weight, blood glucose level, total proteins, hemoglobin, serum albumin, serum urea, serum cholesterol levels were examined in the diabetic rats. The histopathological changes in the pancreas of diabetic rats were also studied. The aqueous extract of Vitis pedata showed a significant reduction in the blood glucose levels, lipid profile and serum biomarkers in diabetic rats, quite similar to the standard treatment of glibenclamide, whereas the ether extract showed a less effect compared to the other two extracts. The plant extracts also highlighted an improvement in the beta cell mass in islets of pancreas. Thus, the study of aqueous and ethanolic extract of Vitis pedata indicated a promising antihyperglycemic activity in alloxan induced diabetic rats. Furthermore, the study also opens the door for extended research to explicate the mechanism of action of the plant extracts.