Evaluation of anticancer activity of methanolic extract of Hiptage benghalensis (L.) Kurz on Cancer Cell Lines

Abstract

Aim and Background: This study aimed to study the anticancer effects of Hiptage benghalensis (L.) kurz using various human cancer cell cultures, in vitro using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. Materials and Methods: Human cervical carcinoma (HeLa) cells, human breast cancer (MCF-7) cells, and human neuroblastoma (IMR-32) cells were maintained in a 5% CO2 incubator at 37°C. Different concentrations of an extract of H. benghalensis in serum-free culture medium were freshly prepared and used for cytotoxic activity using MTT assay activity. Results: Among the plant extract had revealed that, the greater percentage inhibition in all types of cancer cells in a dose-dependent manner using MTT assay. The concentration of the extract causing 50% cell death values of methanolic extract of H. benghalensis were found to be 50.73, 47.90, and 53.76 μg/mL against HeLa, MCF-7, and IMR-32, respectively. Conclusion: H. benghalensis methanolic extract were showed increased percentage inhibition of MCF 7, HeLa, and IMR 32 cells using MTT assay. Abbreviation Used: HBM: Hiptage benghalensis Methanolic extract, MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide, MCF 7: Human breast cancer cells, HeLa: Human cervical carcinoma cells, IMR 32: Human neuroblastoma, H2DCFDA: 2',7' dichlorodihydrofluorescein diacetate, PBS: Phosphate-buffered saline, DMSO: Dimethyl sulfoxide, DCF: Dichlorofluorescein, ROS: Reactive oxygen species.