Evaluation of anticancer activity of leaves of Rumex vesicarius Linn and Symplocos racemosa Roxb. by brine shrimp lethality and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) methods

Abstract

Introduction: The objective of the present study was to evaluate the anticancer activity of leaves of Rumex vesicarius Linn and Symplocos racemosa Roxb. Materials and Methods: In this study, cytotoxicity was assayed by brine shrimp lethality bioassay (BSL) and (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Result: Different extracts of leaves of plant R. vesicarius Linn. and S. racemosa Roxb were found to be cytotoxic to HT-29 and PC-3 cell lines. Different extracts of leaves of plant R. vesicarius Linn. and S. racemosa Roxb showed significant (P < 0.0001) cytotoxic effect on HT-29 and PC-3 cell lines in a dose-dependent manner. It also showed significant cytotoxic effect in BSL bioassay. Conclusion: The study demonstrated that all the extracts of R. vesicarius Linn and S. racemosa Roxb show a significant cytotoxic effect on HT-29 and PC-3 cell lines as well as on BSL bioassay in a dose-dependent manner.