Abstract
The effectiveness of 9 antibiotics against mono- and polymicrobial biofilms formed by Vibrio cholerae O1 strains was studied in vitro on fragments of chitinous exoskeleton of broad- fingered crayfish Astacus astacus in monoculture and together with opportunistic bacteria Klebsiella spp. and non-O1/non-O139 V.cholerae. An increase in the antibiotic resistance of monomicrobial biofilms compared with planktonic bacteria was observed. Resistance of the polymicrobial biofilm bacteria to most antibacterial drugs corresponded to the resistance of the most stable strain in the monomicrobial biofilm. An increase in resistance to two and three drugs was observed in biofilms formed by classical toxigenic strains together with non-O1/non-O139 V.cholerae, while in biofilms formed together with Klebsiella spp. — to one antibacterial drug. Changes in antibiotic sensitivity during the interaction between bacteria in polymicrobial biofilm should be taken into account when developing tactics for prevention and treatment of infections.
Highlights
Èçó÷åíà ýôôåêòèâíîñòü 9 àíòèáèîòèêîâ â îòíîøåíèè ìîíî- è ïîëèìèêðîáíûõ áèîïëíîê, îáðàçîâàííûõ in vitro íà ôðàãìåíòàõ ýêçîñêåëåòà õèòèíîâîãî ïàíöèðÿ øèðîêîïàëîãî ðå÷íîãî ðàêà Astacus astacus øòàììàìè Vibrio cholerae Î1 â ìîíîêóëüòóðå è â àññîöèàöèè ñ óñëîâíî-ïàòîãåííûìè áàêòåðèÿìè Klebsiella spp. è V.cholerae nonO1/nonO139
An increase in resistance to two and three drugs was observed in biofilms formed by classical toxigenic strains together with non-O1/non-O139 V.cholerae, while in biofilms formed together with Klebsiella spp. — to one antibacterial drug
Èññëåäîâàíèÿ ó÷ ̧íûõ ïîêàçàëè, ÷òî ïîëèìèêðîáíûå áèîïëíêè â ñðàâíåíèè ñ ìîíîìèêðîáíûìè èìåþò ïîâûøåííûé óðîâåíü ñèíòåçà ôàêòîðîâ âèðóëåíòíîñòè è áîëåå ïëîòíûé, íåïðîíèöàåìûé äëÿ àíòèáàêòåðèàëüíûõ âåùåñòâ ìàòðèêñ, ñîñòàâ êîòîðîãî ñèëüíî âàðüèðóåò â çàâèñèìîñòè îò âèäà áàêòåðèé è óñëîâèé îêðóæàþùåé ñðåäû, è ñîîòâåòñòâåííî, ìîæåò îáåñïå÷èòü áîëåå ñèëüíóþ çàùèòó ïðîòèâ àíòèìèêðîáíûõ ñðåäñòâ [12]
Summary
Èçó÷åíà ýôôåêòèâíîñòü 9 àíòèáèîòèêîâ â îòíîøåíèè ìîíî- è ïîëèìèêðîáíûõ áèîïëíîê, îáðàçîâàííûõ in vitro íà ôðàãìåíòàõ ýêçîñêåëåòà õèòèíîâîãî ïàíöèðÿ øèðîêîïàëîãî ðå÷íîãî ðàêà Astacus astacus øòàììàìè Vibrio cholerae Î1 â ìîíîêóëüòóðå è â àññîöèàöèè ñ óñëîâíî-ïàòîãåííûìè áàêòåðèÿìè Klebsiella spp. è V.cholerae nonO1/nonO139. An increase in resistance to two and three drugs was observed in biofilms formed by classical toxigenic strains together with non-O1/non-O139 V.cholerae, while in biofilms formed together with Klebsiella spp. Èññëåäîâàíèÿ ó÷ ̧íûõ ïîêàçàëè, ÷òî ïîëèìèêðîáíûå áèîïëíêè â ñðàâíåíèè ñ ìîíîìèêðîáíûìè èìåþò ïîâûøåííûé óðîâåíü ñèíòåçà ôàêòîðîâ âèðóëåíòíîñòè è áîëåå ïëîòíûé, íåïðîíèöàåìûé äëÿ àíòèáàêòåðèàëüíûõ âåùåñòâ ìàòðèêñ, ñîñòàâ êîòîðîãî ñèëüíî âàðüèðóåò â çàâèñèìîñòè îò âèäà áàêòåðèé è óñëîâèé îêðóæàþùåé ñðåäû, è ñîîòâåòñòâåííî, ìîæåò îáåñïå÷èòü áîëåå ñèëüíóþ çàùèòó ïðîòèâ àíòèìèêðîáíûõ ñðåäñòâ [12].
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