Abstract
Background and Objectives: Klebsiella pneumoniae is a gram negative opportunistic pathogen that causes different infections, but in recent years the emergence of resistance to carbapenem antibiotic among these isolates causes failure in the treatment of these infections. The aim of this study was to evaluate the prevalence of carbapenemase producing Klebsiella pneumoniae strains among different wards and various clinical specimens in Isfahan. Material and Method: In this cross sectional study, 100 different clinical samples were collected from different wards of teaching hospitals in Isfahan. K. pneumonia isolates were identified by different standard biochemical tests. Antimicrobial susceptibility tests were performed as standard disk-diffusion based on the instructions of Clinical Laboratory Standards Institute (CLSI). For detection of KPC-producing strains, isolates were investigated by The Modified Hodge Test based on CLSI instruction. Results: The population study was included 62% females and 38% males (p=0.01). The highest and the lowest rate of resistance were observed for piperacillin (84%) and ertapenem (50%) respectively. The Modified Hodge Test was positive for 68 (68%) isolates that the highest rate of resistance was observed for piperacillin (91/2%) and cefotaxime (83/8%) . Conclusion: This study demonstrates the high prevalence of carbapenemase producing Klebsiella pneumoniae isolates ,Which shows an urgent need to review and modify the pattern of antibiotic consumption. In addition, In the later studies genotypic methods for all carbapenemase genes should be performed to determine the cause of the resistance.
Highlights
Klebsiella pneumoniae is a gram-negative opportunistic pathogen that causes different infections, in recent years the emergence of resistance to carbapenem antibiotic among these isolates has caused failure in the treatment of these infections
The aim of this study was to evaluate the prevalence of carbapenemase-producing K. pneumoniae strains among various clinical specimens obtained from different wards of Isfahan hospitals, Iran
K. pneumoniae isolates were detected by IMVIC standard biochemical tests and urease and confirmed by detection of ureD gene, responsible for urea hydrolysis, by polymerase chain reaction (PCR) method
Summary
Klebsiella pneumoniae is a gram-negative opportunistic pathogen that causes different infections, in recent years the emergence of resistance to carbapenem antibiotic among these isolates has caused failure in the treatment of these infections. Objective: The aim of this study was to evaluate the prevalence of carbapenemase-producing K. pneumoniae strains among various clinical specimens obtained from different wards of Isfahan hospitals, Iran. For detection of K. pneumoniae carbapenemase (KPC)-producing strains, isolates were investigated by the modified Hodge test (MHT) based on CLSI instructions. France, Sweden, Norway, Scotland, China, Colombia, Brazil, Trinidad and Tobago, and Poland are the countries that have reported pathogen-harbouring KPCs.[10, 11] Information on this issue is limited in our country
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.