Abstract
BackgroundBacteria like E. faecalis can produce intra- and extra-radicular biofilms. Theoretically, the adjustable penetration ability of lasers enables better access to root canal system. Therefore the aim of the present study was to compare the ability of photoactivated laser and 2.5% NaOCl irrigation solution to eliminate E. faecalis from the root canals by real-time PCR technique.Material and MethodsSixty extracted human upper central incisors were selected and sterilized in an autoclave. The root canals were infected with E. faecalis (PTCC 1237, Persian Type Culture Collection, Iran) and then incubated for 24 hours. The samples were randomly divided into 3 groups. No intervention was made in the control group (group 1). In group 2, laser therapy was performed with a power of 100 mW by diode laser for 120 seconds. In group 3, the canals were irrigated with 5 mL of 2.5% NaOCl; then all the samples were sonicated in 15 mL of normal saline in test tubes in order to isolate the bacteria. DNA extraction was performed followed by real-time PCR technique for all the samples.ResultsInhibition of bacterial growth in all the experimental samples was significantly more than that in the control group. There was a significant difference between photodynamic therapy and 2.5% NaOCl. The effect of NaOCl in all the samples was better than photodynamic therapy. The results of the mean CT (cyclic threshold) were 40, 30.2 and 15.35 for 2.5% NaOCl, photodynamic therapy and control group, respectively.ConclusionsBased on the results of this experimental study, 2.5% NaOCl eliminated E. faecalis from infected root canals more effectively compared to photodynamic therapy. Key words:Photoactivated laser, Enterococcus faecalis, antibacterial agents, sodium hypochlorite.
Highlights
A large number of microorganisms, including E. faecalis, have an important role in the etiology of periradicular lesions after root canal treatment [1,2]
Since no studies to date have used real-time PCR in this respect, this study was designed to compare the capacity of photoactivated laser technique in eliminating E. faecalis from the root canal system in comparison to irrigation with 2.5% NaOCl solution
The results of Tukey test showed a significant difference in mean CT between the photodynamic therapy and 2.5% NaOCl groups, with higher mean CT in the 2.5% NaOCl group compared to the photodynamic therapy group (P
Summary
A large number of microorganisms, including E. faecalis, have an important role in the etiology of periradicular lesions after root canal treatment [1,2]. A new technique for the elimination of microorganisms from the root canal system is the use of low-level laser beams [7] In this technique, light-sensitive materials such as tolonium chloride are used. Molecular techniques have been introduced for identification of microorganisms with various advantages, including detection of cultivable and uncultivable species, high accuracy and specificity, high sensitivity, no need for controlling anaerobic conditions, the capacity to carry it out during antimicrobial therapy, easy transfer of the samples to the laboratory and detection of nonviable microorganisms, this last case is considered an advantage and a disadvantage. The aim of the present study was to compare the ability of photoactivated laser and 2.5% NaOCl irrigation solution to eliminate E. faecalis from the root canals by real-time PCR technique. Conclusions: Based on the results of this experimental study, 2.5% NaOCl eliminated E. faecalis from infected root canals more effectively compared to photodynamic therapy
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