Abstract

This study investigated the antibacterial effect of Diode Laser (810nm) at different output powers in the root canals infected with Enterococcus faecalis in vitro and in vivo study. Root canal specimens were prepared from extracted, 50 single rooted teeth. After root canal preparation and sterilization, the root canals were infected with endodontic pathogens (Enterococcus faecalis). Following laser therapy, the canal contents were cultured on Enterococcus agar. The colony forming units recovered from each root canal was evaluated before and after laser treatment. This study also investigated antibacterial effect of laser in root canals associated with preiradicular lesions in vivo. In this in vivo study, 30 patients with infected root canals underwent diode laser treatment. To verify the findings, microbiological tests were performed and the results were computed. Result showed in vitro significant antibacterial difference between 1.05W, 1.5W, 1.95W and control group. In vivo there was no significant antimicrobial difference between untreated control group and 1.05W but significantly different from 1,5W and 1.95W in which there was no significant different between them.

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