Evaluation of antibacterial effect of silver nanoparticles paste with and without curcumin as intracanal medication

Soha Adel Abdou,Amira Ibrahim Mohamed

doi:10.1186/s42269-022-00723-1

Abstract

BackgroundThe purpose of the current study was to compare the antibacterial activity of silver nanoparticles AgNPs paste and silver nanoparticles AgNPs with curcumin paste as intracanal medications versus calcium hydroxide Ca(OH)2 paste. Thirty extracted, human teeth with single roots were decoronated and mechanically prepared. After sterilization, roots were inoculated with Enterococcus faecalis for 10 days. Roots were separated into three groups in accordance to kind of intracanal medications used; Group A, silver nanoparticles AgNPs (Nanogate company, Cairo, Egypt). Group B, silver nanoparticles AgNPs with curcumin (Nanogate company, Cairo, Egypt). Group C, calcium hydroxide Ca(OH)2 (Metapaste, META BIOMED). First microbiological samples (S1) were collected from the canal, before insertion of intracanal medication. Intracanal medications were kept in root canals in all groups for 7 days; then second microbiological samples (S2) were collected from the canals after medicament removal. After the colony forming units CFUs have been counted, the data were statistically analyzed.ResultsIn the three tested groups, the highest bacterial count was found in (S1), while the least bacterial count was found in (S2) with statistical significant difference between them.ConclusionsThe effect of AgNPs paste and AgNPs with curcumin paste was superior to Ca(OH)2 paste. AgNPs with curcumin paste had the best antibacterial effect.

Highlights

The purpose of the current study was to compare the antibacterial activity of silver nanoparticles Silver nanoparticles (AgNPs) paste and silver nanoparticles AgNPs with curcumin paste as intracanal medications versus calcium hydroxide Ca(OH)2 paste
The most popular used intracanal medicament is calcium hydroxide Ca(OH)2 due to Its high pH 12.5, which has an eradicative effect on proteins structure and cell membrane of the microorganisms (Spangberg et al 1994), but there is several microorganisms that are resistant to it such as Enterococcus faecalis which withstand the vigor of Ca(OH)2 by liberation proton pump which decrease the internal pH of the cell (Haapasalo and Ørstavik 1987)
A statistically significant difference was reported between Ca(OH)2 (Group C) and each of silver nanoparticles AgNPs (Group A) and silver nanoparticles AgNPs with curcumin (Group B) where (p = 0.007) and (p = 0.007), respectively (Table 2)

Summary

Introduction

The purpose of the current study was to compare the antibacterial activity of silver nanoparticles AgNPs paste and silver nanoparticles AgNPs with curcumin paste as intracanal medications versus calcium hydroxide Ca(OH) paste. Roots were separated into three groups in accordance to kind of intracanal medications used; Group A, silver nanoparticles AgNPs (Nanogate company, Cairo, Egypt). Group B, silver nanoparticles AgNPs with curcumin (Nanogate company, Cairo, Egypt). Intracanal medications were kept in root canals in all groups for 7 days; second microbiological samples (S2) were collected from the canals after medicament removal. Instrumentation and irrigation are not sufficient for complete eradication of all remaining microbes in the spaces root canals due to its complexity and presence of resistant microorganisms such as Enterococcus Faecalis, The most popular used intracanal medicament is calcium hydroxide Ca(OH) due to Its high pH 12.5, which has an eradicative effect on proteins structure and cell membrane of the microorganisms (Spangberg et al 1994), but there is several microorganisms that are resistant to it such as Enterococcus faecalis which withstand the vigor of Ca(OH) by liberation proton pump which decrease the internal pH of the cell (Haapasalo and Ørstavik 1987). It has low toxicity as well as accelerates wound healing (Atiyeh et al 2007; Tian et al 2007)

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