Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp.

Abstract

Use of silver nanoparticles to manage pathogenic microorganisms is a modern trend in nanomedicine. Thus our study focused on utilizing the nanoparticle synthesizing properties of marine bacteria. Molecular identification of the selected bacterial strain was done by 16SrDNA sequencing based method, which showed it as a novel Pseudomonas strain. The biosynthesis of silver nanoparticles was obtained by treating the bacteria with 1mM AgNO3 and the isolate was found to have the ability to form silver nanoparticles intracellularly within 24 hours at room temperature. The silver nanoparticles synthesized by the novel isolate were characterized by UV-Vis spectroscopy and scanning electron microscope. The UV-Vis absorption analysis showed a peak at 430 nm corresponding to the surface plasmon resonance of silver nanoparticles. Also these silver nanoparticles were evaluated for their antibacterial efficacy against Salmonella typhi, Vibrio cholerae, Bacillus subtilis and Staphylococcus aureus.