Abstract
Arthritis is a chronic inflammatory condition that is a major disability throughout the world. For so many years there are several therapies used for the treatment but they have many disadvantages like side effects, poor bioavailability, high cost etc. So, researches are going on for the development of alternative effective therapy for arthritis. The current study explores anti-arthritic activity (by targeting pro-inflammatory cytokines like IL-6)and phytochemical analysis of different fractions of Capsicum annum, Boswellia serrata and Lawsonia inermis.In this study overall seven fractions were prepared with the help of fractionation. They were coded as CO,COHF, COMF, BPS, HMEP, HMAP and HAMF. The anti- inflammatory activity of the fractions was evaluated by using an in-vitro IL-6 and TNF-ɑ ELISA study. The non-toxic concentration of the study samples was determined by MTT assay. High-PerformanceThin-Layer Chromatography was used for qualitative analysis of phytoconstituents present in the fractions. Liquid Chromatography-Mass Spectroscopy was used for the identification of phytoconstituents present in the fractions of COMF, BPS and HMEP. HPTLC analysis revealed the presence of capsaicin, galactose, arabinose, luteolin, β-sitosterol, LCMS analysis showed the presence of capsaicin, luteolin, lalioside and arabinose
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