Evaluation of anti-aging klotho protein in COPD rehabilitation

Abstract

Introduction: The α-klotho protein has been implicated in longevity and its plasma level was reported to be modulated by skeletal muscle activity, physical fitness, nutritional status and chronic stress. As pulmonary rehabilitation generally improves these physiological factors, we hypothesised that a complex rehabilitation program may alter plasma klotho in patients with COPD. Aims: We aimed to study whether the clinical response of patients is reflected by a change in plasma klotho level, and whether the initial concentration of the protein can predict the effectivity of the rehabilitation program. Methods: Blood samples were taken from 19 COPD patients (63.3±8.8 years (mean±SD), FEV 1 35.7±10.7%) at the beginning and at the end of a 3-week inpatient pulmonary rehabilitation program. Plasma klotho level was measured using ELISA. Clinical parameters (respiratory function, 6MWD, COPD Assessment Test (CAT), degree of dyspnea (mMRC test), grip strength, chest expansion and breath holding time) were measured before and after rehabilitation. Results: 6MWD (373.7±123.5 vs. 414.5±118.6 m, p 0.05). Subgroup analysis revealed no difference in klotho between patients who improved in any of the above parameters vs. those who did not. Conclusion: Despite clinical improvement, plasma klotho concentration remains stable during rehabilitation. Plasma klotho concentration does not have prognostic value for the responsivity of COPD patients.