Evaluation of anti-arthritic activity of Spathodea Campanulata of root bark extract on freund’s adjuvant induced arthritis in rats models

Abstract

Rheumatoid arthritis is a chronic systemic inflammation of the synovial joint and progressive destruction of cartilage and bone that may affect many tissues and organs-skin, blood vessels, heart, lungs and muscles but principally attacks the joints, producing a non-suppurative proliferative andinflammatory synovitis that often progresses to destruction of the articular cartilage and ankylosis of the joints. Angiogenesis and microvascular lesions are common features of RA inflammation, which leads to abnormal serum protein infiltration into the synovium. The objective of the study was to determine the preliminary phytochemical constituents, to evaluate acute toxicity studies, In-vitroanti-oxidant studies, In vitroand In-vivoanti-arthritic activity of theroot bark of Spathodea campanulata.. Paw edema was induced in rats by intra plantar injection of 0.1ml of Complete Freund’s Adjuvant (CFA) in left hind paw. The ethanolic extract of Spathodea campanulata (150mg/Kg & 300mg/Kg) and Diclofenac Sodium were orally administered for 28 consecutive days.Percentage inhibition was determined by Protein denaturation method and Membrane Stabilizing Activity Hematological, Histopathology, Radiological Analysis of CFA-induced arthritis in rat was performed. Statisticalsignificant was analyzed by one-way ANOVA followed by Tukey test. Our study concluded that ethanolic extract at the dose of 300 mg/kg showed significant anti-arthritic activity when compared to Diclofenac sodium