Evaluation of anthelmintic activity of dryopteris filix-mas using earthworm

Abstract

Evaluation of anthelmintic activity of a leaves extracts of Dryopteris filix-mas against earthworm with Preliminary work as physical characteristics. Finally extraction of defatted Dryopteris filix-mas extract was done with hydro-alcoholic solvent and % yield was found to be 6.55% w/w and their characteristics are reported. Results obtained from qualitative chemical tests are tabulated. Total alkaloid content was calculated as atropine equivalent mg/100 mg using the equation based on the calibration curve: Y=0.007X+ 0.007, R2=0.999, where X is the Atropine equivalent (AE) and Y is the absorbance. Total phenol content was expressed as mg/100 mg of gallic acid equivalent of dry extract sample using the equation obtained from the calibration curve: Y = 0.017X+0.017, R2= 0.998, where X is the gallic acid equivalent (GAE) and Y is the absorbance. The present findings indicated that the usefulness of the hydroalcoholic extract of leaves of Dryopteris filix-mas in earthworm. This study suggested, hydroalcoholic extract of leaves of Dryopteris filix-mas dose- dependently produced anthelmintic activity.