Evaluation of an ovarian synchronization scheme for fixed-time artificial insemination in wapiti

Abstract

The ovarian response to an empirically derived treatment protocol used commercially for fixed-time insemination in wapiti ( Cervus elaphus) was evaluated by transrectal ultrasonography in hinds during transition into the ovulatory season. On September 29, hinds ( n = 7) were given an intravaginal progesterone-releasing device (CIDR-B, 1.9 g of progesterone) or left untreated (controls, n = 9). Fourteen days later, hinds in the treated group were given 200 IU eCG and the CIDR was removed. Hinds in the control group ovulated randomly over a 15 day period. In the treated group, five hinds ovulated 3 days after eCG treatment, one ovulated 7 days after treatment, and one failed to ovulate by November 1. All extant dominant follicles ceased growth and/or began to regress within 2 days of CIDR placement. Two waves of follicular development were detected between CIDR insertion and removal; the first emerged 5.1 ± 0.5 days after CIDR insertion and the second at 11.0 ± 0.7 days. Serum progesterone concentration was 0.6 ± 0.5 ng/mL (range 1.0–0.3 ng/mL) before CIDR placement, remained above 6 ng/mL during CIDR placement, and fell to 0.8 ± 0.9 ng/mL after CIDR removal. In the control group, maximal luteal-phase progesterone concentration was lower (1.1 ± 0.1 ng/mL; P < 0.05) and emergence of the first follicular wave was more variable ( P = 0.05) than in the treated group. The protocol to synchronize ovulation was effective in 5/7 (71%) hinds, and 4/7 (57%) became pregnant and calved. The pregnancy rate (6/9) and calving rate (5/9) was similar in the control group. In conclusion, synchronization with CIDR-B was effective; however, the protocol may be improved by shortening the interval of CIDR placement to ≤7 days and by reducing the circulating concentrations of progesterone to physiologic concentrations (<4 ng/mL).