Evaluation of an autogenous vaccine against E. coli bearing the CTX-M-14 plasmid Roberto M. La Ragione, Sabrina Lotz, Hannah Cockrem, Martin J. Woodward and Nick Coldham Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (Weybridge), UK.

Abstract

Event Abstract Back to Event Evaluation of an autogenous vaccine against E. coli bearing the CTX-M-14 plasmid Roberto La Ragione1* 1 University of Surrey, Veterinary Laboratories Agency - Department of Bacteriology, United Kingdom Roberto M. La Ragione, Sabrina Lotz, Hannah Cockrem, Martin J. Woodward and Nick Coldham Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (Weybridge), UK. Over the past decade enteric bacteria in Europe, Africa, and Asia have become increasingly resistant to cephalosporin antibiotics. This is largely due to the spread of genes encoding extended-spectrum beta-lactamase (ESBL) enzymes that can inactivate 3rd and 4th generation cephalosporins. The recent emergence of bacteria carrying ESBLs has highlighted their zoonotic potential and the requirement to prevent their dissemination through the food chain. One such approach to controlling bacterial disease is the use of autogenous vaccines. Autogenous vaccines are manufactured from the specific pathogenic bacteria isolated from the diseased animal. The studies presented here aimed to establish if there is any scientific evidence to support the use of autogenous vaccines to prevent the colonisation of cattle by ESBL harbouring bacteria. Preliminary studies established a bovine colonisation and persistence model for E. coli O33 bearing the blaCTX-M-14 plasmid. An autogenous vaccine study was then set up whereby thirty 5-6 week old cross bred calves were randomly divided into two groups, one receiving the vaccine and the other a placebo intramuscularly. At 12 weeks of age all calves were orally challenged with 1 x 1010 CFU of E. coli O33 and faecal shedding monitored daily. Total E. coli and E. coli O33 shedding rates were determined using selective media. Serial post-mortem examinations were also performed to evaluate colonisation levels together with detailed histopathological studies. Blood samples were taken throughout the study for immunological assays. The results indicated good colonisation of the bovine GIT by the ESBL bearing E. coli O33. However, there was no significant protection afforded by the autogenous vaccine as assessed by faecal shedding and enumeration of ESBLs from tissues sampled at post mortem examination. Interestingly though, an elevated immune response to E. coli O33 was detected in the serum of the vaccinated animals. Future studies aim to evaluate the effect of the vaccine on plasmid transfer and the identification of ESBL specific antigens that may be appropriate as novel vaccine candidates. Keywords: Autogenous vaccine, Calves, ESBL harbouring bacteria Conference: ECMIS - E. coli and the Mucosal Immune System : Interaction, Modulation and Vaccination, Ghent, Belgium, 2 Jul - 5 Jul, 2011. Presentation Type: Oral Presentation Topic: Abstracts Citation: La Ragione R (2012). Evaluation of an autogenous vaccine against E. coli bearing the CTX-M-14 plasmid . Front. Immunol. Conference Abstract: ECMIS - E. coli and the Mucosal Immune System : Interaction, Modulation and Vaccination. doi: 10.3389/conf.fimmu.2012.01.00010 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 09 Jan 2012; Published Online: 12 Jan 2012. * Correspondence: Prof. Roberto La Ragione, University of Surrey, Veterinary Laboratories Agency - Department of Bacteriology, Surrey, GU2 7XH, United Kingdom, R.Laragione@surrey.ac.uk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Roberto La Ragione Google Roberto La Ragione Google Scholar Roberto La Ragione PubMed Roberto La Ragione Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.