Abstract
Two widely applied spectrophotometric assays based on aluminium complex formation used for determination of total flavonoid content in food or medicinal plant samples were examined for several compounds from different classes of flavonoid family. The method which involves the measurement at 410–430 nm after addition of AlCl3 solution is selective only for flavonols and flavones luteolin. The procedure in the presence of NaNO2 in alkaline medium seems to be specific for rutin, luteolin and catechins, but also phenolic acids exhibit considerable absorbance at 510 nm. Application of both procedures to natural samples gave different order in terms of their flavonoid content. Thus, the expression “total flavonoid” content is not adequate as the results of both methods are dependent on the structure of the individual flavonoids present.
Highlights
The spectrophotometric assay based on aluminium complex formation is one of the most commonly used procedure for the so-called total flavonoid determination, as the content of these compounds is considered as an important parameter for evaluating food or medicinal plant samples
In the literature data for determination of total flavonoid content, procedure 1 was applied in the presence of acid, acetate salts or without addition of any reagent (Table 1)
Spectrophotometric assay based on aluminium complex formation is one of the most commonly applied procedure for determination of total flavonoid content in food or medicinal plant samples
Summary
The spectrophotometric assay based on aluminium complex formation is one of the most commonly used procedure for the so-called total flavonoid determination, as the content of these compounds is considered as an important parameter for evaluating food or medicinal plant samples. This method, proposed initially by Christ and Müller (1960) for the analysis of herbal materials, was latter several times modified. Careful research in literature indicated variation in experimental conditions upon application of Al-flavonoid complexation reaction. The observation and suggestions was tested for several commercial samples
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