Abstract
Accumulation of pesticide due to its overuse and recalcitrant property is a major problem owing to its well-established toxicity in living systems, thus creating a need for designing strategies for in-situ detection and monitoring. Enzymatic biosensors provide advantages over conventional chromatographic detection methods in terms of cost, time, labour and on-site detection. Current research trends on enzymatic biosensors for major pesticide groups; organophosphates, organochlorine and carbamates mostly focus on the compound’s target enzyme inhibition for detection. In this study, we have identified novel enzymes from microbial pesticide degradation systems, which show activity on the pesticides Atrazine, DDT or Methyl parathion. The binding affinities of the enzymes to their respective pesticide substrates were evaluated based on computational docking studies. These enzymes show potential for being used as receptors for catalytic based biosensors for pesticide detection.
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