Evaluation of agricultural soil health after applying pyraclostrobin in wheat/maize rotation field based on the response of soil microbes

Abstract

The green development of agriculture, the rational use of pesticides, and soil health are closely related. However, our understanding of them is still limited. Pyraclostrobin (PYRA) is one of the most popular strobilurin fungicides due to its broad-spectrum control of fungal diseases. However, PYRA has been shown to have negative effects on bacterial communities and soil enzyme activities under laboratory conditions. The effects of PYRA on soil microbes (mainly bacteria) in actual field environments still need to be addressed. In the present study, after applying PYRA in a wheat/maize rotation field for 2 years, we determined the level of residual PYRA and assessed the soil bacterial community structure, soil enzyme activity, and ammonia-oxidizing functional gene expression. The results show that the DT50 of PYRA in agricultural soil was 2.16–38.93 days. The final residual levels of PYRA in wheat/maize grain were less than 0.01 mg kg−1, which is lower than the maximum residue limit (MRL) recommended (0.2 mg kg−1) by China and the European Union. The culturable fungi and bacteria populations were significantly reduced after applying PYRA. The genera’s abundance (Sphingomonas and Lysobacter) and urease activity associated with phosphorus cycle were inhibited. The amoA genes abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) showed that the N-cycle function of soil microbes was inhibited. Overall, applying PYRA for 2 years did not increase the negative response of soil microbes. However, in some indicators, such as culturable microorganism populations, more significant reductions in populations were observed in the double recommended dose group than in the recommended dose group. The results obtained in this study reflect the response of soil microbes subjected to pyraclostrobin in an actual field and provide new insights into how agricultural development influences soil health.