Evaluation of Aflatoxin B1 Binding Capacity with Mix Toxin Binder using Central Composite Design

Abstract

Aflatoxin B1 (afl B1) binding capacity of a mixed toxin binder used in poultry nutrition were determined using the central composite design technique. Experimental conditions such as pH, temperature and incubation time for the determination of the binding capacity of a mix binder were optimized for Central Composite Design. The impact of these three independent variables on the % binding of aflatoxin B1 was evaluated at different five levels (-1.68, -1, 0, 1, 1.68). The optimum experimental conditions were 5.8, 42°C, 94.11 min for pH, temperature and incubation time, respectively using quadratic model and desirability function. A significant effect of each independent variable was observed on the % binding efficiency of aflatoxin B1. In optimum experimental conditions, aflatoxin B1 binding capacity with mix toxin binder was found 97%. The results of the present study indicated that the mix binder is very suitable for binding of aflatoxin B1 and the central composite design can be used effectively in determining the optimized parameters for improving toxin binding capacity of aflatoxin B1.