Evaluation of affinity interaction between drugs and platelet by open tubular capillary electrochromatography

Abstract

An open tubular capillary electrochromatography (CEC) method was developed for the evaluation of affinity interaction between drugs and platelet. The platelet was attached onto the inner surface of the capillary by physical adsorption, while the coated length was controlled by varying the injection time of platelet suspension. According to the magnitude of the apparent mobility of the analytes and the coated length of platelet, as well as the chromatographic distribution theory of CEC, the equation for calculating of binding constant was deduced. By using the developed method, the binding constants of the anti-platelet aggregation drug aspirin and the negative control pazufloxacin were determined. The results indicated the strong interaction between aspirin and platelet with the binding constant of 0.579 g^-1 L, while the pazufloxacin and platelet showed weak interaction with the binding constant of 0.249 g^-1 L. In the end, affinity extraction has been used for contrast. It can be concluded that the developed method can be used for the determination of affinity interaction of drug with platelet as well as other living cell that can be coated onto the inner surface of capillary.